Vaxcyte’s Research team uses a broad palette of chromatographic tools to purify diverse vaccine antigens. Applicants must have practical, hands-on experience with multiple chromatographic modalities (not limited to monoclonal antibody/Protein A workflows). Experience should include selection, screening, development, scale-up and characterization of various chromatography techniques.
- Execute the following AKTA FPLC chromatography techniques:
Immobilized metal affinity chromatography (IMAC)
Ion-exchange chromatography (IEX)
Hydrophobic interaction chromatography (HIC)
Size-exclusion chromatography (SEC)
Hydroxyapatite (CHT / HA)
- Designing chromatography trains to reduce host cell protein, endotoxin and aggregates using complementary modalities.
- Demonstrated DoE experience for multimodal optimization is a plus.
- Membrane chromatography for high-throughput contaminant removal or virus/VLP processing and as a scale-friendly polishing/scavenging step.
- Keep accurate and current records of development experiments and/or project related activities in an electronic laboratory notebook.
- Present data from experiments to the team and contribute to technical discussions.
- Work within a team environment and provides support as necessary to further the team’s initiatives.
- BS in Biochemistry, Molecular Biology or a relevant science considered, with minimum of 2 years of relevant industry experience or MS with minimum of 1 year of relevant industry experience.
- Experience with diverse protein targets, bacterial and eukaryotic recombinant proteins, small/large subunit vaccine antigens, fusion proteins, multimeric complexes, glycoproteins, and VLPs.
- The role requires demonstrated success with alternative capture/polishing methods and orthogonal cleaning strategies appropriate for potential vaccine antigens.
- Preferred experience with Cell-free protein synthesis system.
- Familiarity with automation or high throughput workflows such as (96-well plates) and UNICORN/AKTA software DoE integrations.
- Experience in process development.
- Strong interpersonal skills to collaborate well within the team and excellent written and verbal communication skills. Reports to: Scientist II, Research Location: San Carlos, CA Work Arrangement: Onsite Expected Contract Length: 12months Hourly Range: $51/hr.
- $56/hr. We are an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability or veteran status.